Relaxin Test Kit
Relaxin Test Kit
The Breeders Cupboard

Relaxin Test Kit

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Confirm your female's pregnancy with this one-step rapid Relaxin test kit.

Each test kit comes complete with a syringe, pipette, test tube and test.

The test will require blood collection and must be separated into serum using a centrifuge.  Relaxin test results are available within a few minutes with  95% accuracy.

Pregnant females produce a hormone called relaxin during the developing stage of the placenta. At around 22 - 27 days post-implantation relaxin can be detected in the female's bloodstream and the remaining elevated majority of the pregnancy up until birth.

I recommend waiting until 30 days post ovulation to run a relaxin test. Please also remember that if you have a pregnancy that is reabsorbing there can still be relaxin present. So a repeat test may be valuable. 

Available in a single, pack of 2 tests & 5 pack of Tests

**** If you are not able to do a blood collection or spin the blood down with a centrifuge there is an alternative that is very easy to use Belly Labs ***

here is a link

Can the relaxin test tell the difference between pregnancy and pseudopregnancy?

Pseudopregnancy (pseudo = false) is a trick of nature that causes a female dog to look and behave as if she is pregnant even though she is not. The signs appear for about two months immediately after a heat period.

There is no actual placental development during pseudopregnancy, and relaxin is not produced at any time. The relaxin test will always be negative in the pseudopregnant dog and can be used reliably to tell the difference between pregnancy and pseudopregnancy.

Does a single negative relaxin test mean a female dog is not pregnant?

Any negative result may indicate that a female dog is not pregnant. However, a false negative result may occur if the test is performed too early in pregnancy, before the placenta has started to produce relaxin. A dog that is negative for relaxin on the initial test, performed at 22-27 days post-breeding, should be tested again one week later to confirm the negative results. Doing multiple tests is especially important in the early stages of pregnancy and whenever the breeding dates are unknown.

"A false negative result may occur if the test is performed too early in pregnancy."

In most cases (80% of the time), two consecutive negative relaxin tests one week apart, starting 22-27 days post-breeding, confirm that a dog is not pregnant. Rarely, a third test at 31-34 days post-breeding may be needed if breeding occurred very early in the heat period. There is also evidence that dogs of small and toy breeds may not produce enough relaxin to turn the test positive, especially if they are carrying only one or two puppies. In these females, alternative testing is used to determine if puppies are present (see below).

Does a positive relaxin test mean a dog is pregnant?

A positive relaxin test indicates that a dog is pregnant at the time of the test - conception has occurred, implantation of an embryo has taken place, and a placenta is developing.

Does a positive relaxin test mean that live puppies will be born?

Not necessarily. A positive relaxin test indicates only that a dog is pregnant at the time of the test. It does not predict that the pregnancy will end successfully with the delivery of live puppies.

 Unfortunately, there are many reasons why pregnancies fail, and fetuses can be lost at any stage after conception, up to and including whelping. Sometimes a dog will be positive for relaxin on the first test, but will be negative on a later test. This indicates that the pregnancy has been lost, even though the dog may not have shown any sign of a problem.

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